熊本大学大学院生命科学研究部 

2003年以前

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【著書】
・ 松下正之,富澤一仁,松井秀樹.蛋白質の細胞内導入法。必ず上手くいく遺伝子導入と発現解析プロトコール.中嶋一範・北村義浩編 実験医学別冊 (羊土社)71-78, 2003
・ 富澤一仁、松井秀樹.Cdk5, Cdk5 activator (p35nck5a, p35).ノックアウトマウス・データブック.黒川清・笹月健彦監.Molecular Medicine 臨時増刊号:(中山書店)97-98, 1997.

【総説】
・ Matsui, H., Tomizawa, K., Lu, Y.-F., and Matsushita, M. Protein Therapy: In vivo protein transduction by polyarginine (11R) PTD and subcellular targeting delivery. Curr. Protein Pept. Sci. 4: 151-157, 2003.
・ 松井秀樹,富澤一仁,松下正之.タンパク質セラピー法:Poly-Arginineによる非ウイルス的タンパク質細胞内導入と細胞内微小環境への局在化法.日本薬理学雑誌 121: 435-439, 2003.
・ 富澤一仁,松井秀樹.オキシトシンの新しい生理機能.医学のあゆみ 206: 225-226, 2003.
・ 富澤一仁,松下正之,松井秀樹.アルギニンによる細胞内蛋白質輸送.栄養ー評価と治療 20: 59-63, 2003.
・ 松下正之, 富澤一仁, 李勝天, 森脇晃義, 松井秀樹.蛋白質導入法による神経可塑性研究.日本生理学雑誌64: 12-14, 2002.
・ 富澤一仁、松下正之、松井秀樹、杉本直己. 遺伝子治療からタンパク質セラピーへ。化学 57: 50-55, 2002.
・ 松下正之、富澤一仁、李 勝天、森脇晃義、松井秀樹.ポリアルギニンによるリン酸化阻害ペプチドの神経細胞内への導入.脳の科学 23:798-800、2001.
・ 松井秀樹、富澤一仁、松下正之、森脇晃義.脳の可塑性におけるカルシニューリンの役割.医学のあゆみ 193:945-949、2000.

【原著論文】
・ Tomizawa, K., Iga, N., Lu, Y.-F., Moriwaki, A., Matsushita, M., Li, S.-T., Miyamoto, O., Itano, T., and Matsui, H. Oxytocin improves long-lasting spatial memory during motherhood through MAP kinase cascade. Nat. Neurosci. 6: 384-390, 2003.
・ Tomizawa, K., Sunada, S., Lu, Y.-F., Oda, Y., Kinuta, M., Ohshima, T., Saito, T., Wei, F.-Y., Matsushita, M., Li, S.-T., Tsutsui, K., Hisanaga, S., Mikoshiba, K., Takei, K., and Matsui, H. Cophosphorylation of amphiphysin I and dynamin I by Cdk5 regulates clathrin-mediated endocytosis of synaptic vesicles. J. Cell Biol. 163: 813-824, 2003.
・ Terada, H., Matsushita, M., Lu, Y.-F., Shirai, T., Li, S.-T., Tomizawa, K., Moriwaki, A., Nishio, S., Date, I., Ohmoto, T., and Matsui, H. Inhibition of excitatory neuronal cell death by cell permeable calcineurin autoinhibitory peptide. J. Neurochem. 87: 1145-1151, 2003.
・ Nozaki, K., Tomizawa, K., Yokoyama, N., Kumon, H., and Matsui, H. Calcineurin mediates bladder smooth muscle hypertrophy after bladder outlet obstruction. J. Urol. 170: 2077-2081, 2003.
・ Wu, H.-Y., Tomizawa, K., Matsushita, M., Lu, Y.-F., Li, S.-T., and Matsui, H. Poly-arginine-fused calpastatin peptide, a living cell membrane-permeable and specific inhibitor for calpain. Neurosci. Res. 47: 131-135, 2003.
・ Yamashita, S., Miyamoto, O., Janjua, N., Tomizawa, K., Matsui, H., Nakamura, T., Nagao, S., and Itano, T. Role of hippocampal CA2 region following postischemic hypothermia in gerbil. Mol. Brain Res. 111: 8-16, 2003.
・ Sato K., Nishina Y., Shiga K., & Tanaka F. Hydrogen-bonding dynamics of free flavins in benzene and FAD in electron-transferring flavoprotein upon excitation. Journal of Photochemistry & Photobiology. B—Biology. 70(2), 67–73, 2003
・ Sato K., Nishina Y., & Shiga K. Purification of electron-transferring flavoprotein from Megasphaera elsdenii and binding of additional FAD with an unusual absorption spectrum. Journal of Biochemistry. 134(5), 719–729, 2003
・ Nishina Y., Sato K., Tamaoki H., Tanaka T., Setoyama C., Miura R., & Shiga K. Molecular mechanism of the drop in the pKa of a substrate analog bound to medium-chain acyl-CoA dehydrogenase: implications for substrate activation. Journal of Biochemistry. 134(6), 835–842, 2003
・ Tomizawa, K., Ohta J, Matsushita M, Moriwaki A, Li S-T, Takei K., and Matsui H. Cdk5/p35 regulates neurotransmitter release through phosphorylation and downregulation of P/Q-type voltage-dependent calcium channel activity. J. Neurosci. 22: 2590-2597, 2002.
・ Li, S.-T., Kato, K, Tomizawa, K., Matsushita, M., Moriwaki, A., Matsui, H., and Mikoshiba, K. Calcineurin plays different roles in group II metabotropic glutamate receptor- and NMDA receptor-dependent long-term depression. J. Neurosci. 22: 5034-5041, 2002.
・ Takenobu, T., Tomizawa, K., Matsushita, M., Li, S.-T., Moriwaki, A., Lu, Y.-F., and Matsui, H. Development of p53 protein transduction therapy using membrane-permeable peptides and the application to oral cancer cells. Mol. Cancer Ther. 1: 1043-1049, 2002.
・ Matsushita, M., Tomizawa, K., Moriwaki, M., Li, S-H., Terada, H., Ohmoto, T., and Matsui, H. A high efficiency protein transduction system proving the role of PKA in long lasting LTP. J. Neurosci. 21: 6000-6007, 2001.
・ Nishina Y., Sato K., Shi R., Setoyama C., Miura R., & Shiga K. On the ligands in charge-transfer complexes of porcine kidney flavoenzyme D-amino acid oxidase in three redox states: a resonance Raman study. Journal of Biochemistry. 130(5), 637–647, 2001
・ Tomizawa, K., Cai, X-H., Moriwaki, A., Matsushita, M., and Matsui, H. Involvement of Cyclin-dependent kinase 5/p35nck5a in the synaptic reorganization of the rat hippocampus during  kindling progression. Jpn. J. Physiol. 50: 525-532, 2000.
・ Feng, J., Yan, Z., Ferreira, A., Tomizawa, K., Liauw, J.A., Zhuo, M., Allen P.B., Ouimet, C.C., and Greengard P. Spinophilin regulates the formation and function of dendritic spines. Proc. Natl. Acad. Sci. USA 97: 9287-9292, 2000.
・ Yan, Z., Hsieh-Wilson, L., Feng, J., Tomizawa, K., Allen, P. B., Feinberg, A. A., Nairn, A. C., and Greengard, P. Protein phosphatase 1 modulation of neostriatal AMPA channels: regulation by DARPP-32 and spinophilin. Nat. Neurosci. 2: 13-17, 1999.
・ Lu, Y.-F., Kojima, N., Tomizawa, K., Moriwaki, A., Matsushita, M. Obata, K., and Matsui, H. Enhanced synaptic transmission and reduced threshold for LTP induction in fyn-transgenic mice. Eur. J. Neurosci. 11: 75-82, 1999.
・ Musa, F. R., Tokuda, M., Kuwata, Y., Ogawa, T., Tomizawa, K., Konishi, R., Takenaka, I. and Hatase, O. Expression of cyclin-dependent kinase 5 and associated cyclins in Leydig and Sertoli cells of the testis. J. Androl. 19: 657-666, 1998.
・ Ogawa, T., Tokuda, M., Tomizawa, K., Matsui, H., Itano, T., Konishi, R., Nagahata, S., and Hatase, O. Osteoblastic differentiation is enhanced by rapamycin in rat osreoblast-like osteosarcoma (ROS 17/2.8) cells. BBiochem. iophys. Res. Commun. 249: 226-230, 1998.
・ Onuma, H., Lu, Y.-F., Tomizawa, K. Moriwaki, A., Tokuda, M., Hatase, O., and Matsui, H. A calcineurin inhibitor, FK506, blocks voltage-gated calcium channel-dependent LTP in the hippocampus. Neurosci. Res. 30: 313-319, 1998.
・ Moriwaki, A., Lu, Y.-F., Tomizawa, K. and Matsui, H. An immunosuppressant, FK506, protects against neuronal dysfunction and death but has no effect on electrographic and behavioral activities induced by systemic kainate. Neuroscience 86: 855-865, 1998.
・ Matsui, H., Lu, Y.-F., Tomizawa, K., and Moriwaki, A. Calcineurin inhibitors, FK506 and Cyclosporin A, modulate neuronal plasticity. Kinase and phosphatase in lymphocyte and neuronal signaling: 262-271, 1997.
・ Lu, Y.-F., Moriwaki, A., Tomizawa, K., and Matsui, H. Effect of vasopressin and involvement of receptor subtypes in the rat amygdaloid nucleus in vitro. Brain Res. 768: 266-272, 1997.
・ Cai, X.-H., Tomizawa, K., Tang, D., Lu, Y.-F., Moriwaki, A., Tokuda, M., Nagahata, S., Hatase, O., and Matsui, H. Changes in the expression of novel Cdk5 activator messenger RNA (p39nck5ai) during rat brain development. Neurosci. Res. 28: 355-360, 1997.
・ Moriwaki, A., Lu, Y.-F., Hayashi, Y., Tomizawa, K., Tokuda, M., Itano, T., Hatase, O., and Matsui, H. Immunosuppressant FK506 prevents mossy fiber sprouting induced by kindling stimulation. Neurosci. Res. 25: 191-194, 1996.
・ Lu, Y.-F., Moriwaki, A., Hayashi, Y., Tomizawa, K., and Matsui, H. Effects of neurotensin on neurons in the rat central amygdaloid nucleus in vitro. Brain Res. Bull. 40: 135-141, 1996.
・ Lu, Y.-F., Hayashi, Y., Moriwaki, A., Tomizawa, K., and Matsui, H. FK506, a Ca2+/calmodulin-dependent phosphatase inhibitor, inhibits the induction of long-term potentiation in the rat hippocampus. Neurosci. Lett. 205: 103-106, 1996.
・ Lu, Y.-F., Tomizawa, K., Moriwaki, A., Hayashi, Y., Tokuda, M., Itano, T., Hatase, O., and Matsui, H. Calcineurin inhibitors, FK506 and  cyclosporin A, suppress the NMDA receptor-mediated potentials and LTP, but not depotentiation in the rat hippocampus. Brain Res. 729: 142-146, 1996.
・ Matsushita, M., Tomizawa, K., Lu, Y.-F., Moriwaki, A., Tokuda, M., Itano, T., Wang, J.H., Hatase, O., and Matsui, H. Distinct subcellular localization of cyclin dependent  kinase (Cdk) 5 and neuron- specific Cdk5 activator protein (p35nck5a) in the developing rat cerebellum. Brain Res. 734: 319-322, 1996.
・ Tomizawa, K., Matsui, H., Matsushita, M. Tokuda, M., Itano, T., Konishi, R., Lew, J., Wang, J.H., and Hatase, O. Localization and developmental changes of the novel brain specific cyclin (p35nck5a) in the rat brain. Neuroscience 74: 519-529, 1996.
・ Hirooka, K., Tomizawa, K., Matsui, H., Tokuda, M., Itano, T., Hasegawa, E., Wang, J.H., and Hatase, O. Developmental alteration of the expression and kinase activity of cyclin-dependent kinase5 (Cdk5)/p35nck5a in the rat retina. J. Neurochem. 67: 2478-2483, 1996.
・ Tang, D., Yeung, J., Li, K.Y., Matsushita, M., Matsui, H., Tomizawa, K., Hatase, O., and Wang, J. H. An isoform of neuronal cyclin-dependent kinase 5 (Cdk5) activator. J. Biol. Chem. 270: 26897-26903, 1995.
・ Tomizawa, K., Matsui, H., Kondo, E., Miyamoto, K., Tokuda, M., Itano T.,  Nagahata, S., Akagi, T., and Hatase, O. Developmental alteration and neuron-specific expression of bone morphogenetic protein (BMP) – 6 mRNA in rodent brain. Mol. Brain Res. 28: 122-128. 1995.
・ Matsushita. M., Matsui, H., Itano, T., Tomizawa, K., Tokuda, M., Suwaki, H., Wang, J.H., and Hatase, O. Developmental changes of cyclin dependent kinase 5 subcellular localization in the rat cerebellum. NeuroReport 6: 1267-1270, 1995.
・ Miyamoto, K., Matsui, H., Tomizawa, K., Kuwata, Y., Itano, T., Tokuda, M., and Hatase, O. In situ localization of rat testis-specific calcineurin B subunit isoform 1 in the developing rat testis. Biochem. Biophys. Res. Commun. 203: 1275-1283, 1994.
・ Moia, L.J.M.P., Matsui, H., Barros, G.A.M.de, Tomizawa, K., Miyamoto, K., Kuwata, Y., Tokuda, M., Itano T., and Hatase, O. Immunosuppressants and calcineurin inhibitors, cyclosporin A and FK506, reversibly inhibit epileptogenesis in amygdaloid kindled rat. Brain Res. 648: 337-341, 1994.
・ Nishina Y., Sato K., Miura R., & Shiga K. Substrate recognition and activation mechanism of D-amino acid oxidase: a study using substrate analogs. Journal of Biochemistry. 128(2), 213–223, 2000
・ Sato K., Nishina Y., Setoyama C., Miura R., & Shiga K. Unusually high standard redox potential of acrylyl-CoA/propionyl-CoA couple among enoyl-CoA/acyl-CoA couples: a reason for the distinct metabolic pathway of propionyl-CoA from longer acyl-CoAs. Journal of Biochemistry. 126(4), 668–675, 1999
・ Nishina Y., Sato K., Miura R., Matsui K., & Shiga K. Resonance Raman study on reduced flavin in purple intermediate of flavoenzyme: use of [4-carbonyl-18O]-enriched flavin. Journal of Biochemistry. 124(1), 200–208, 1998
・ Hazekawa I., Nishina Y., Sato K., Shichiri M., Miura R., & Shiga K. A Raman study on the C(4)=O stretching mode of flavins in flavoenzymes: hydrogen bonding at the C(4)=O moiety. Journal of Biochemistry. 121(6), 1147–1154, 1997
・ Sato K., Nishina Y., & Shiga K. In vitro refolding and unfolding of subunits of electron-transferring flavoprotein: characterization of the folding intermediates and the effects of FAD and AMP on the folding reaction. Journal of Biochemistry. 120(2), 276–285, 1996
・ Nishina Y., Sato K., Miura R., & Shiga K. Structures of charge-transfer complexes of flavoenzyme D-amino acid oxidase: a study by resonance Raman spectroscopy and extended Hückel molecular orbital method. Journal of Biochemistry. 118(3), 614–620, 1995
・ Hazekawa I., Nishina Y., Sato K., Shichiri M., & Shiga K. Substrate activating mechanism of short-chain acyl-CoA, medium-chain acyl-CoA, long-chain acyl-CoA, and isovaleryl-CoA dehydrogenases from bovine liver: a resonance Raman study on the 3-ketoacyl-CoA complexes. Journal of Biochemistry. 118(5), 900–910, 1995
・ Nishina Y., Sato K., Hazekawa I., & Shiga K. Structural modulation of 2-enoyl-CoA bound to reduced acyl-CoA dehydrogenases: a resonance Raman study of a catalytic intermediate. Journal of Biochemistry. 117(4), 800–808, 1995
・ Sato K., Nishina Y., & Shiga K. Preparation of separated alpha and beta subunits of electron-transferring flavoprotein in unfolded forms and their restoration to the native holoprotein form. Journal of Biochemistry. 116(1), 147–155, 1994
・ Miura R., Nishina Y., Sato K., Fujii S., Kuroda K., & Shiga K. 13C- and 15N-NMR studies on medium-chain acyl-CoA dehydrogenase reconstituted with 13C- and 15N-enriched flavin adenine dinucleotide. Journal of Biochemistry. 113(1), 106–113, 1993
・ Sato K., Nishina Y., & Shiga K. Electron-transferring flavoprotein has an AMP-binding site in addition to the FAD-binding site. Journal of Biochemistry. 114(2), 215–222, 1993
・ Sato K., Nishina Y., & Shiga K. Anion-induced conformational change of apo–electron-transferring flavoprotein. Journal of Biochemistry. 111(3), 359–365, 1992
・ Nishina Y., Sato K., Shiga K., Fujii S., Kuroda K., & Miura R. Resonance Raman study on complexes of medium-chain acyl-CoA dehydrogenase. Journal of Biochemistry. 111(6), 699–706, 1992
・ Sato K., Nishina Y., & Shiga K. The binding of adenine nucleotides to apo–electron-transferring flavoprotein. Journal of Biochemistry. 112(6), 804–810, 1992
・ Nishina Y., Sato K., & Shiga K. Isomerization of Δ1-piperideine-2-carboxylate to Δ2-piperideine-2-carboxylate on complexation with flavoprotein D-amino acid oxidase. Journal of Biochemistry. 109(5), 705–710, 1991
・ Sato K., Nishina Y., Shiga K., Tojo H., & Tada M. The existence of two different forms of apo–electron-transferring flavoprotein. Journal of Biochemistry. 109(5), 734–740, 1991
・ Suzuki H., Koyama H., Nishina Y., Sato K., & Shiga K. A resonance Raman study on a reaction intermediate of Pseudomonas L-phenylalanine oxidase (deaminating and decarboxylating). Journal of Biochemistry. 110(2), 169–172, 1991
・ Nishina Y., Sato K., & Shiga K. Proton release from flavoprotein D-amino acid oxidase on complexation with the zwitterionic ligand, trigonelline. Journal of Biochemistry. 107(5), 726–731, 1990

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